Python sequences are indexed with positive numbers and negative numbers. For positive numbers 0 is the first index 1 is the second index and so forth. For negative indices -1 is the last index and -2 is the penultimate (next to last) index and so forth. Think of seq[-n] as the same as seq[len(seq)-n].
Using negative indices can be very convenient. For example S[:-1] is all of the string except for its last character, which is useful for removing the trailing newline from a string.